odhA [2019-08-05 09:19:52]

2-oxoglutarate dehydrogenase (E1 subunit)

locus: BSU_19370

pI: 5.87

mw: 105.54 kDa

protein length: 944 aa

gene length: 2835 bp

function: TCA cycle

product: 2-oxoglutarate dehydrogenase (E1 subunit)

essential: no

ec: 1.2.4.2

synonyms: citK

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.4|TCA cycle]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

Gene

Coordinates: 2,108,774 2,111,608

The protein

Catalyzed reaction/ biological activity

• 2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine --> [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO₂ (according to UniProt)

Protein family

• alpha-ketoglutarate dehydrogenase family (single member, according to UniProt)

Modification

• phosphorylated on Arg-66 and Arg-621 [Pubmed|22517742]

Structure

• [PDB|2JGD] (from ''Escherichia coli'', 38% identity, 55% similarity) [Pubmed|17367808]

[SW|Localization]

• cytoplasm (according to UniProt)

Additional information

• extensive information on the structure and enzymatic properties of 2-oxoglutarate dehydrogenase can be found at [http://www.proteopedia.org/wiki/index.php/2-Oxoglutarate_Dehydrogenase Proteopedia]

Expression and Regulation

Operons

• genes: [gene|3B54AD8514709655BA829347B7BD5B38EF7C08ED|odhA]-[gene|02BA02D10DFB06E51101D8CF76BCF5BED94D7CA2|odhB]
  description: [Pubmed|11976317]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1508153], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|12850135], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • repressed by glucose (2.4-fold) ([protein|search|CcpA]) [Pubmed|12850135]

Biological materials

Mutant

• GP671 (spc), GP684 (cat), available in [SW|Jörg Stülke]'s lab
• GP1274 Δ([gene|3B54AD8514709655BA829347B7BD5B38EF7C08ED|odhA])::''cat'', available in [SW|Jörg Stülke]'s lab
• GP1276 Δ([gene|3B54AD8514709655BA829347B7BD5B38EF7C08ED|odhA]-[gene|02BA02D10DFB06E51101D8CF76BCF5BED94D7CA2|odhB])::''cat'', available in [SW|Jörg Stülke]'s lab
• GP2183 Δ([gene|3B54AD8514709655BA829347B7BD5B38EF7C08ED|odhA])::''ermC'', available in [SW|Jörg Stülke]'s lab [pubmed|28679749]
• GP2332 Δ([gene|3B54AD8514709655BA829347B7BD5B38EF7C08ED|odhA]-[gene|02BA02D10DFB06E51101D8CF76BCF5BED94D7CA2|odhB])::''kan'', Cre-recombinase is integrated in ''[gene|8C2DD87E0351BBEE0FD8C0462087CCAD7459AA88|sacA]'', available in [SW|Jörg Stülke]'s lab
• GP2334 Δ([gene|3B54AD8514709655BA829347B7BD5B38EF7C08ED|odhA]-[gene|02BA02D10DFB06E51101D8CF76BCF5BED94D7CA2|odhB])::''lox72'', Cre-recombinase is integrated in ''[gene|8C2DD87E0351BBEE0FD8C0462087CCAD7459AA88|sacA]'', available in [SW|Jörg Stülke]'s lab
• BKE19370 ([gene|3B54AD8514709655BA829347B7BD5B38EF7C08ED|odhA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGAATATTACCCCCAA, downstream forward: _UP4_AAAAACTAAGGGGGAAATGA
• BKK19370 ([gene|3B54AD8514709655BA829347B7BD5B38EF7C08ED|odhA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGAATATTACCCCCAA, downstream forward: _UP4_AAAAACTAAGGGGGAAATGA

lacZ fusion

• pGP3106 (in [SW|pAC7]), available in [SW|Jörg Stülke]'s lab

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

References

Reviews

• 10672230

Original publications

• 2500417,20933603,1508153,11976317,12850135,22517742,22900538,24263382